@article{Ecker2006,
	title = {An improved method for discrimination of cell populations in tissue sections using microscopy-based multicolor tissue cytometry},
	volume = {{69A}},
	url = {http://dx.doi.org/10.1002/cyto.a.20219},
	doi = {10.1002/cyto.a.20219},
	abstract = {In tissue context, researchers and pathologists lack a generally applicable standard for quantitative determination of cytological parameters. Increasing knowledge of disease-specific markers calls for an appropriate in situ tissue {cytometry.Microscopy-based} multicolor tissue cytometry {(MMTC)} permits multicolor analysis of single cells within tissue {context.Tissue} specimens stained for {CD45/CD3/CD4/CD8} were analyzed. Specificity as well as reproducibility of {MMTC} is demonstrated and a novel {MMTC-based} function to improve visual discrimination of subpopulations is {introduced.Our} data demonstrate that {MMTC} constitutes an important step toward automated and quantitative fluorometry of solid tissues and cell monolayers. © 2006 International Society for Analytical Cytology},
	number = {3},
	journal = {Cytometry Part A},
	author = {Rupert C. Ecker and Radu Rogojanu and Marc Streit and Katja Oesterreicher and Georg E. Steiner},
	year = {2006},
	pages = {119--123}
}