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Automatic image aquisition of embedded materials

Three-dimensional image acquisition technologies which are capable of digitizing the 3D structure of materials are developing rapidly. Especially in the field of medical science these techniques are well investigated. Versatile methods are available for 3D digitization, which roughly can be divided into two main groups destructive and non-destructive. Confocal laser scanning microscopy (CLSM), X-ray microtomography, optical coherence tomography (OCT), nuclear magnetic resonance (NMR) and ultrasonic microscopy are the most popular in the field of non destructive techniques, whereas sheet splitting and serial sectioning are the most common destructive methods.

In cooperation with the Institute of Paper, Fiber and Pulp Technology at Graz University of Technology (http://www.ipz.tugraz.at), a novel 3D image acquisition system which enables 3D digitization of embedded materials with high resolution, sufficient sample size and moderate operating time and costs was developed. A prototype which combines serial sectioning and light microscopy was designed and built. The design of the developed prototype is shown below. The approach allows 3D digitization in a fully automated way without any need for user interaction. The applicability of the prototype was mainly shown for digitization of paper samples, but in general the methods can be used for digitizing various kinds of materials, thus applications in the field of medical histology, biology or polymer industry seem to be very promising.

Automated Microtomy Concept

Publications

  • Kontschieder, P., Donoser, M. and H.Bischof. (2010) Detecting Paper Fibre Cross Sections in Microtomy Images. In Proceedings of International Conference on Pattern Recognition (ICPR), Istanbul, Turkey.
  • Eckhart, R., Donoser, M., and Bauer, W. (2009). Single Fibre Flexibility Measurement in a Flow Cell Based Device. Fundamental Research Symposium - Advances in Pulp and Paper Research.
  • Hirn, U., Donoser, M., Kritzinger, J. and W.Bauer. (2009). Introducing a Concept to Link {3D} Paper Structure to {2D} Paper Properties. Fundamental Research Symposium - Advances in Pulp and Paper Research.
  • Kritzinger, J., Donoser, M. and Bauer, W. (2009). Representative Analysis of Fibre Cross Section Properties Using Automated Microtomy. Fundamental Research Symposium - Advances in Pulp and Paper Research.
  • Donoser, M., Mauthner, T., Kritzinger, J. and Bischof, H. (2008). A Probabilistic Approach for Tracking Fibers. In Proceedings of International Conference on Pattern Recognition (ICPR), Tampa, USA.
  • Donoser, M., Wiltsche, M., and Bischof, H. (2005). A new automated microtomy concept for 3D paper structure analysis. In Proceedings of Conference on Machine Vision Applications (MVA), pages 76–79, Tsukuba, Japan.
  • Donoser, M., Wiltsche, M., Bischof, H., and Bauer W. (2005). Paper coating layer analysis based on computer vision methods. In Proceedings of International Conference on Quality Control by Artificial Vision (QCAV), pages 39–44, Nagoya, Japan.
  • Wiltsche, M., Donoser, M., Bauer W., and Bischof, H. (2005). A new slicebased concept for 3D paper structure analysis applied to spatial coating layer formation. In Proceedings of Advances in Paper Science and Technology (FRC), pages 853–899, Cambridge, UK.
  • Wiltsche, M., Bauer, W., and Donoser, M. (2006). Coating application method and calendering influence on the spatial coating layer formation obtained by an automated serial sectioning method. In Proceedings of TAPPI Advanced Coating Fundamentals Symposium, pages 413-–425, Turku, Finland, Invited Publication.
  • Wiltsche, M., Donoser M., and Bauer, W. (2006) Tracking of fibers in 3D paper structures obtained by an automated microtomy method. In Proceedings of Progress in Paper Physics Conference, pages 68–72.
  • Wiltsche, M., Donoser, M., and Bauer, W. (2006) First applications of a novel technique for three-dimensional coating layer analysis. In Proceedings of PTS Coating Symposium, pages 39.1–39.15, Baden-Baden, Germany.
  • Wiltsche, M., Donoser, M., and Bauer, W. (2006). A novel destructive approach for 3D paper structure analysis. Lenzinger Berichte, 86:90–95.
  • Wiltsche, M. and Donoser, M. (2004). Die räumliche Strichdickenverteilung als Anwendung der dreidimensionalen Papierstrukturanalyse. Wochenblatt für Papierfabrikation, 132(18):1082—1088.
  • Wiltsche, M. and Donoser, M. (2004). Dreidimensionale Strukturanalyse in Papier. In Österreichische Papierfachtagung, Graz (Austria).
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